Status | Page | User | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
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unacceptable | NEUCR:V5IKE7 | Obeykimb, MichSt14A 41 | 2014-04-05 17:31:38 CDT | GO:0004707 MAP kinase activity (F) | PMID:19884508 | ECO:0000315 mutant phenotype evidence used in manual assertion | Figure 1: Mutations in the MAPK gene mak-2 result in strains that are blind; mak-2 mutants fail to undergo chemotropic interactions and vegetative cell fusion, even when interacting with wild-type cells. To determine the spatiotemporal localization dynamics of MAK-2 during chemotropic interactions, live-cell imaging was performed on genetically identical germlings containing a functional GFP tagged MAK-2 protein. The mutants showed no chemotropic interactions and had the GFP labeled protein evenly distributed through out the cell and the non mutants showed migration of the GFP labeled protein accumulated in the cell cortex.
| challenge |
unacceptable | 9BIVA:M9QW24 | Obeykimb, MichSt14A 41 | 2014-04-03 17:01:45 CDT | GO:0070167 regulation of biomineral tissue development (P) | PMID:23732290 | ECO:0000314 direct assay evidence used in manual assertion | Figure 5 shows the relative expression level of perlucin in mantle after shell damage. Within the first 2 hours after shell damage the level of perlucin is more than double the level in the undamaged control providing proof this protein plays a major role in biomineral tissue development.
| challenge |
requireschanges | BACIU:Q7SID8 | Obeykimb, MichSt14A 41 | 2014-04-03 16:37:53 CDT | GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds (F) | PMID:20730475 | ECO:0000314 direct assay evidence used in manual assertion | The recombinant Xyn10 can hydrolyze xylan from
oatspelt and birchwood, but cannot degrade methyl
cellulose, citrus fruits pectin, maize starch, locust
bean gum, or maize amylopectin (Table 3). Thus,
Xyn10 is a specific xylanase.
| challenge |
acceptable | 9BACI:Q5YEX6 | Obeykimb, MichSt14A 41 | 2014-04-03 16:24:33 CDT | GO:0004567 beta-mannosidase activity (F) | PMID:15316858 | ECO:0000314 direct assay evidence used in manual assertion | This mannanse was examined for its ability to hydrolyze various substrates. This table shows this enzyme was specific toward bB1,4-
mannosidic linkages of mannopolysaccharides
| challenge |
unacceptable | PINNO:CPRM | Obeykimb, MichSt14A 41 | 2014-04-03 16:09:25 CDT | GO:0031215 shell calcification (P) | PMID:15994301 | ECO:0000314 direct assay evidence used in manual assertion | Figure 9A: Shows in vitro inhibition of the precipitation of CaCO3 by caspartin; this protein is dose dependently an inhibitor of shell calcification
| challenge |
acceptable | TELCI:M4TB00 | Obeykimb, MichSt14A 41 | 2014-04-02 21:03:59 CDT | GO:0004586 ornithine decarboxylase activity (F) | PMID:23499950 | ECO:0000315 mutant phenotype evidence used in manual assertion | Table 1: Full length cDNA encoding ornithine decarboxylases were cloned into Teladorsagia circumcincta (TcODC) and Haemonchus contortus (HcODC). Recombineered mutants Km compared to adult H. contortus and T. circumcincta were similar (two other worm homogenates)
| challenge |
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Pages in category "MichSt14A 41"
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