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Category:MichSt14A 41

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableNEUCR:V5IKE7Obeykimb, MichSt14A 412014-04-05 17:31:38 CDTGO:0004707 MAP kinase activity (F)PMID:19884508ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1: Mutations in the MAPK gene mak-2 result in strains that are blind; mak-2 mutants fail to undergo chemotropic interactions and vegetative cell fusion, even when interacting with wild-type cells. To determine the spatiotemporal localization dynamics of MAK-2 during chemotropic interactions, live-cell imaging was performed on genetically identical germlings containing a functional GFP tagged MAK-2 protein. The mutants showed no chemotropic interactions and had the GFP labeled protein evenly distributed through out the cell and the non mutants showed migration of the GFP labeled protein accumulated in the cell cortex.

challenge
unacceptable9BIVA:M9QW24Obeykimb, MichSt14A 412014-04-03 17:01:45 CDTGO:0070167 regulation of biomineral tissue development (P)PMID:23732290ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the relative expression level of perlucin in mantle after shell damage. Within the first 2 hours after shell damage the level of perlucin is more than double the level in the undamaged control providing proof this protein plays a major role in biomineral tissue development.

challenge
requireschangesBACIU:Q7SID8Obeykimb, MichSt14A 412014-04-03 16:37:53 CDTGO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds (F)PMID:20730475ECO:0000314 direct assay evidence used in manual assertion

The recombinant Xyn10 can hydrolyze xylan from

oatspelt and birchwood, but cannot degrade methyl cellulose, citrus fruits pectin, maize starch, locust bean gum, or maize amylopectin (Table 3). Thus, Xyn10 is a specific xylanase.

challenge
acceptable9BACI:Q5YEX6Obeykimb, MichSt14A 412014-04-03 16:24:33 CDTGO:0004567 beta-mannosidase activity (F)PMID:15316858ECO:0000314 direct assay evidence used in manual assertion

This mannanse was examined for its ability to hydrolyze various substrates. This table shows this enzyme was specific toward bB1,4-

mannosidic linkages of mannopolysaccharides

challenge
unacceptablePINNO:CPRMObeykimb, MichSt14A 412014-04-03 16:09:25 CDTGO:0031215 shell calcification (P)PMID:15994301ECO:0000314 direct assay evidence used in manual assertion

Figure 9A: Shows in vitro inhibition of the precipitation of CaCO3 by caspartin; this protein is dose dependently an inhibitor of shell calcification

challenge
acceptableTELCI:M4TB00Obeykimb, MichSt14A 412014-04-02 21:03:59 CDTGO:0004586 ornithine decarboxylase activity (F)PMID:23499950ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1: Full length cDNA encoding ornithine decarboxylases were cloned into Teladorsagia circumcincta (TcODC) and Haemonchus contortus (HcODC). Recombineered mutants Km compared to adult H. contortus and T. circumcincta were similar (two other worm homogenates)

challenge

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Pages in category "MichSt14A 41"

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