GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.

Have any questions? Please email us at ecoliwiki@gmail.com

Category:MichSt14A 40

From GONUTS
Jump to: navigation, search
StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBACSU:MCPCMcfadd48, MichSt14A 402014-04-06 17:55:15 CDTGO:0050918 positive chemotaxis (P)PMID:23038252ECO:0000314 direct assay evidence used in manual assertion

Table 1 shows the results from a capillary assay, indicating that McpC alone can support chemotaxis to 17 amino acids.

challenge
unacceptableARATH:F4IU18Mcfadd48, MichSt14A 402014-04-06 17:44:22 CDTGO:0000302 response to reactive oxygen species (Figure 7 shows that the rol5-1 mutant is hypersensitive to hydrogen peroxide and hyposensitive to the ROS scavenger CuCl2, indicating the importance of ROL5 in the sensing of and response to ROS.)PMID:20530756IMP: Inferred from Mutant Phenotype
challenge
unacceptablePSEAE:Q9HXI7Mcfadd48, MichSt14A 402014-04-06 16:30:36 CDTGO:0006979 response to oxidative stress (P)PMID:24466226ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows decreased survival under oxidative stress conditions in an iscR mutant. Tn7-borne expression of iscR in the iscR mutant restored survival percentage to that of wild-type P. aeruginosa.

challenge
acceptableAEDAE:Q534Q6Mcfadd48, MichSt14A 402014-04-06 13:58:06 CDTGO:0002218 activation of innate immune response (P)PMID:15722339ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4A shows transfection of REL1A alone can induce expression of Cecropin A and Defensin A in Aag2 cells. Figure 4D shows RNAi knockdown of REL1 decreases fungal infection survival in mosquitoes.

challenge
updatedbyinstructorXANOB:G7TJY8Mcfadd48, MichSt14A 402014-04-06 13:13:00 CDTGO:0009405 pathogenesis (P)PMID:24675748ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A shows that a XOC_1550 mutant causes significantly shorter lesions, and Figure 2E shows that XOC_1550 mutant is impaired in its ability to grow in rice, identifying Xrp5 as a critical protein in pathogenesis

challenge
updatedbyinstructorDICD3:E0SG24Mcfadd48, MichSt14A 402014-04-05 23:48:31 CDTGO:0045892 negative regulation of transcription, DNA-templated (P)PMID:22267675ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2A shows that in a slyA mutant, hrp genes controlling the Type 3 Secretion System are overexpressed.

challenge
unacceptableANOGA:B2FXG9Mcfadd48, MichSt14A 402014-04-05 20:31:16 CDTGO:0002218 activation of innate immune response (P)PMID:16076953ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3A shows that silencing of either domain of REL2 results in significant reduction of CEC1 (Cecropin A) expression.

challenge
unacceptableENTFA:Q82ZP8Mcfadd48, MichSt14A 402014-04-04 11:16:34 CDTGO:0033554 cellular response to stress (P)PMID:22092815ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 indicates that a slyA mutant strain is significantly impaired in its ability to grow under bile salt stress (in the presence of 0.08% bile salts), compared to wild-type E. faecalis

challenge
unacceptableENTFA:Q82ZP8Mcfadd48, MichSt14A 402014-04-04 11:16:34 CDTGO:0033554 cellular response to stress (P)PMID:22092815ECO:0000314 direct assay evidence used in manual assertion

Figure 1 shows induction of slyA under bile salt stress conditions.

challenge
acceptableSTRMU:Y1061Mcfadd48, MichSt14A 402014-04-04 11:04:18 CDTGO:0030695 GTPase regulator activity (F)PMID:24659773ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4: An endpoint colorimetric assay indicated that the GTPase activity of Ffh alone, as well as that of Ffh/scRNA, was significantly increased upon addition of YlxM.

challenge

What do the icons mean in the status column?

assessment icons.jpg

Pages in category "MichSt14A 40"

This category contains only the following page.