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Category:GO:0004088 ! carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
name: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
namespace: molecular_function
def: "Catalysis of the reaction: 2 ATP + L-glutamine + CO2 + H2O = 2 ADP + phosphate + glutamate + carbamoyl phosphate." [EC:6.3.5.5]
subset: gosubset_prok
synonym: "carbamoyl phosphate synthase (glutamine-hydrolyzing) activity" EXACT []
synonym: "carbamoyl phosphate synthetase activity" EXACT [EC:6.3.5.5]
synonym: "carbamoyl-phosphate synthase (glutamine-hydrolysing) activity" EXACT [EC:6.3.5.5]
synonym: "carbamoyl-phosphate synthetase (glutamine-hydrolysing) activity" EXACT [EC:6.3.5.5]
synonym: "carbamoyl-phosphate synthetase (glutamine-hydrolyzing) activity" EXACT [EC:6.3.5.5]
synonym: "carbamoylphosphate synthetase II activity" EXACT [EC:6.3.5.5]
synonym: "carbamyl phosphate synthetase (glutamine) activity" EXACT [EC:6.3.5.5]
synonym: "carbon-dioxide::L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating) activity" EXACT [EC:6.3.5.5]
synonym: "CPS activity" EXACT [EC:6.3.5.5]
synonym: "GD-CPSase activity" EXACT [EC:6.3.5.5]
synonym: "glutamine-dependent carbamoyl-phosphate synthase activity" EXACT [EC:6.3.5.5]
synonym: "glutamine-dependent carbamyl phosphate synthetase activity" EXACT [EC:6.3.5.5]
synonym: "hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating) activity" EXACT [EC:6.3.5.5]
xref: EC:6.3.5.5
xref: MetaCyc:CARBPSYN-RXN
xref: Reactome:REACT_102934 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Saccharomyces cerevisiae"
xref: Reactome:REACT_115736 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Gallus gallus"
xref: Reactome:REACT_28976 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Staphylococcus aureus N315"
xref: Reactome:REACT_29452 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Taeniopygia guttata"
xref: Reactome:REACT_30926 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Schizosaccharomyces pombe"
xref: Reactome:REACT_478 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Homo sapiens"
xref: Reactome:REACT_77966 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Bos taurus"
xref: Reactome:REACT_79566 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Canis familiaris"
xref: Reactome:REACT_83511 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Mus musculus"
xref: Reactome:REACT_86599 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Caenorhabditis elegans"
xref: Reactome:REACT_86815 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Plasmodium falciparum"
xref: Reactome:REACT_88920 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Danio rerio"
xref: Reactome:REACT_89432 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Dictyostelium discoideum"
xref: Reactome:REACT_94822 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Drosophila melanogaster"
xref: Reactome:REACT_96943 "L-glutamine + 2 ATP + HCO3- + H2O => carbamoyl phosphate + L-glutamate + 2 ADP + orthophosphate, Rattus norvegicus"
xref: RHEA:24654
is_a: GO:0016884 ! carbon-nitrogen ligase activity, with glutamine as amido-N-donor
AmiGO
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Usage Notes
Because this version of the activity is glutamine-dependent, you should also annotate to GO:0006541_%21_glutamine_metabolic_process
E. coli has a single CPS (carbamoyl phosphate synthetase) encoded by 2 genes, carA and carB. CPS is comprised of a heterodimer of CarA (referred to as the small subunit) and CarB (large subunit). CarA is responsible for binding of glutamine at a conserved catalytic dyad (Cys-His) and initiating the hydrolysis of glutamine to glutamate and ammonia. CarA then transfer of the ammonia group through an ammonia tunnel to the CarB subunit. The large subunit (CarB) then catalyzes an initial round of phosphorylation of bicarbonate to carbamate at the first active site. This product of this reaction travels down the carbamate tunnel where a second phosphorylation reaction of carbamate produces carbamoyl phosphate at the second active site in CarB. CarB is not capable of producing carbamoyl phosphate without CarA in the presence of glutamine. However, CarB can catalyze its production in the presence of ammonia as the nitrogen donor. The next two steps in the synthesis of pyrimidine ribonucleotides are catalyzed by 2 separate enzymes: aspartate transcarbamoylase and dihydroorotase.[1]
Interestingly, higher eukaryotes have multiple CPS enzymes. CAD is a multifunctional protein that encodes CPS function, as well as aspartate transcarbamoylase and dihydroorotase.[1]
References
See Help:References for how to manage references in GONUTS.
Channeling of substrates and intermediates in enzyme-catalyzed reactions. Huang X, Holden HM, Raushel FM. [1]
- ↑ 1.0 1.1 1.2 Huang X et al. (2001) Channeling of substrates and intermediates in enzyme-catalyzed reactions. Annu Rev Biochem 70: 149-80 PubMed GONUTS page
Pages in category "GO:0004088 ! carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"
The following 13 pages are in this category, out of 13 total.
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