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CLOBU:MALQ
Contents |
| Species (Taxon ID) | Clostridium butyricum. ([1]) | |
| Gene Name(s) | malQ | |
| Protein Name(s) | 4-alpha-glucanotransferase
Amylomaltase Disproportionating enzyme D-enzyme | |
| External Links | ||
| EMBL | L37874 | |
| ProteinModelPortal | Q59266 | |
| CAZy | GH77 | |
| BRENDA | 2.4.1.25 | |
| GO | GO:0005737 GO:0004134 GO:0043169 | |
| InterPro | IPR003385 IPR013781 IPR017853 | |
| Gene3D | G3DSA:3.20.20.80 | |
| Pfam | PF02446 | |
| SUPFAM | SSF51445 | |
| TIGRFAMs | TIGR00217 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004134 |
4-alpha-glucanotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004134 |
4-alpha-glucanotransferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded From UniProt |
|||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0016740 |
transferase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0016757 |
transferase activity, transferring glycosyl groups |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0016757 |
transferase activity, transferring glycosyl groups |
IDA: Inferred from Direct Assay |
F |
See figures 3 & 4 |
complete | |||
| GO:0043169 |
cation binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004134 |
4-alpha-glucanotransferase activity |
IDA: Inferred from Direct Assay |
F |
See Figures 3 and 5. Deletion subcloning of its recombinant plasmid indicated that the gene(s) responsible for amylose degradation was localized on a 1.8 kb NspHI-Scal fragment. This region was sequenced in its entirety and shown to encompass a large ORF capable of encoding a protein with a calculated molecular mass of 57,184 Da. Although the deduced amino acid sequence showed only weak similarity with known amylases, significant sequences identity was apparent with the 4-alpha-glucano-transferase enzymes of Streptococcus pneumoniae (46.9%), potato (42.9%) and E. coli (16.2%). The clostridial gene (designated maIQ) was followed by a second ORF which, through its homology to the equivalent enzymes of E. coli and S. pneumoniae, was deduced to encode maltodextrin phosphorylase (MaIP). The translation stop codon of MaIQ overlapped the translation start codon of the putative maIP gene, suggesting that the two genes may be both transcriptionally and translationally coupled. 4-alpha-Glucanotransferase catalyses a disproportionation reaction in which single or multiple glucose units from oligosaccharides are transferred to the 4-hydroxyl group of acceptor sugars. Characterization of the recombinant C. butyricum enzyme demonstrated that glucose, maltose and maltotriose could act as acceptor, whereas of the three only maltotriose could act as donor. The enzyme therefore shares properties with the E. coli MaIQ protein, but differs significantly from the glucanotransferase of Thermotoga maritima, which is unable to use maltotriose as donor or glucose as acceptor. Physiologically, the concerted action of 4-alpha-glucanotransferase and maltodextrin phosphorylase provides C. butyricum with a mechanism of utilizing amylose/maltodextrins with little drain on cellular ATP reserves |
complete | |||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 Goda SK et al. (1997) Molecular analysis of a Clostridium butyricum NCIMB 7423 gene encoding 4-alpha-glucanotransferase and characterization of the recombinant enzyme produced in Escherichia coli. Microbiology 143 ( Pt 10): 3287-94 PubMed GONUTS page
