CANFA:RAB7A

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Species (Taxon ID)	Canis familiaris (Dog) (Canis lupus familiaris). (taxon:9615)
Gene Name(s)	RAB7A ( synonyms: RAB7 )
Protein Name(s)	Ras-related protein Rab-7a
External Links
UniProt Identifier	RAB7A_CANFA
UniProt Accessions	P18067,
EMBL	M35522,
PIR	B30413,
RefSeq	NP_001003316.1,
Ensembl	ENSCAFT00000006672, ENSCAFT00000038482,
Pfam	PF00071,

Annotations

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005770	late endosome		IEA: Inferred from Electronic Annotation		C	Source: UniProtKB-SubCell
	GO:0005764	lysosome		IEA: Inferred from Electronic Annotation		C	Source: UniProtKB-SubCell
	GO:0042470	melanosome		IEA: Inferred from Electronic Annotation		C	Source: UniProtKB-SubCell
	GO:0045335	phagocytic vesicle		IEA: Inferred from Electronic Annotation		C	Source: UniProtKB-SubCell
	GO:0005525	GTP binding		IEA: Inferred from Electronic Annotation		F	Source: UniProtKB-KW
	GO:0015031	protein transport		IEA: Inferred from Electronic Annotation		P	Source: UniProtKB-KW
	GO:0007264	small GTPase mediated signal transduction		IEA: Inferred from Electronic Annotation		P	Source: InterPro
	GO:0005525	GTP binding	PubMed ID:18272684	IDA: Inferred from Direct Assay		F	Figure1A: GTP overlay tested by SDS-page analysis showed that the Rab7 wild type isoform and the active mutant isoforms of the protein where able to bind to the radioactive GTP while the dominant negative mutant, Rab7T22N, (which had a reduced affinity to GTP) didn’t bind since no band was seen in the gel. Figure 1B: Western Blot analysis made with a monoclonal anti-Myc antibody showed the expression of the Myc-tagged Rab7 wild type protein as well.All this indicates that the Rab7 protein binds to GTP.	complete
	GO:0005097	Rab GTPase activator activity	PubMed ID:18272684	IMP: Inferred from Mutant Phenotype		F	Figure 2 A: Mutated protein isoforms showed slower GTPase hydrolysis than the Rab7 wild type.	complete
	GO:0002057	guanine binding	PubMed ID:18272684	IMP: Inferred from Mutant Phenotype		F	Figure 2 B&C: The figures demonstrating the measurement of the GTPase activity of the His-tagged proteins show that the wild type Rab7 protein had a high affinity for guanine nucleotides specifically when GDP was added compared to the mutated Rab7 proteins associated with Charcot Marie Tooth Disease Type 2B	complete
	GO:0005515	protein binding	PubMed ID:18272684	IPI: Inferred from Physical Interaction	UniProtKB:Q96NA2	F	Figure. 4 : Using two hybrid assays ;the wild type of Rab7 and the CMT2B-associated Rab7 mutants were interacting strongly with RILP .Also Rab7 Q67L (which is an active mutated Rab7 protein impaired in GTP hydrolysis not GTP binding ) showed a strong interaction with RILP compared to the interaction of the dominant-negative mutant Rab7 T22N which was extremely weak .	complete
	GO:0005770	late endosome	PubMed ID:18272684	IDA: Inferred from Direct Assay		C	(Figure. 6M–X): When co-expressed with RILP,the Rab7 wild type, Rab7 Q67L and the CMT2B-associated Rab7 mutant proteins were present on the clustered endosomal structures. Which indicates that Rab7 not only interacts with RILP but it also colocalizes with RILP.	complete
	GO:0000166	nucleotide binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F
	GO:0003924	GTPase activity	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	F
	GO:0005525	GTP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001806	F
	GO:0005525	GTP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003579	F
	GO:0005525	GTP binding	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005225	F
	GO:0005525	GTP binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0342	F
	GO:0005525	GTP binding	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	F
	GO:0005764	lysosome	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0458	C
	GO:0005764	lysosome	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	C
	GO:0005764	lysosome	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0158	C
	GO:0005768	endosome	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0967	C
	GO:0005770	late endosome	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSMUSP00000109230	C
	GO:0005770	late endosome	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	C
	GO:0005770	late endosome	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0152	C
	GO:0005794	Golgi apparatus	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSMUSP00000109230	C
	GO:0005794	Golgi apparatus	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	C
	GO:0006184	GTP catabolic process	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	P
	GO:0006810	transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P
	GO:0007174	epidermal growth factor catabolic process	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	P
	GO:0007264	small GTPase mediated signal transduction	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001806	P
	GO:0007264	small GTPase mediated signal transduction	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003579	P
	GO:0008333	endosome to lysosome transport	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	P
	GO:0015031	protein transport	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003579	P
	GO:0015031	protein transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0653	P
	GO:0019003	GDP binding	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	F
	GO:0031410	cytoplasmic vesicle	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0968	C
	GO:0042470	melanosome	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0161	C
	GO:0043231	intracellular membrane-bounded organelle	GO_REF:0000019	IEA: Inferred from Electronic Annotation	Ensembl:ENSP00000265062	C
	GO:0045335	phagocytic vesicle	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0206	C
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CANFA:RAB7A

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