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BACSU:BGAL2
Contents |
| Species (Taxon ID) | Bacillus subtilis. (1423) | |
| Gene Name(s) | ganA (synonyms: galO, lacA, yvfN) | |
| Protein Name(s) | Beta-galactosidase ganA
Beta-gal Beta-1,4-galactooligomerase Galactooligomerase | |
| External Links | ||
| UniProt | O07012 | |
| EMBL | Z94043 AL009126 | |
| PIR | B69649 | |
| RefSeq | NP_391293.1 | |
| ProteinModelPortal | O07012 | |
| SMR | O07012 | |
| CAZy | GH42 | |
| EnsemblBacteria | EBBACT00000001327 | |
| GeneID | 936313 | |
| GenomeReviews | AL009126_GR | |
| KEGG | bsu:BSU34130 | |
| NMPDR | fig|224308.1.peg.3419 | |
| PATRIC | 18978828 | |
| GenoList | BSU34130 | |
| GeneTree | EBGT00050000006111 | |
| HOGENOM | HBG476453 | |
| PhylomeDB | O07012 | |
| ProtClustDB | CLSK537148 | |
| BioCyc | BSUB:BSU34130-MONOMER | |
| GO | GO:0009341 GO:0004565 GO:0046872 GO:0005975 | |
| InterPro | IPR013739 IPR013738 IPR013780 IPR003476 IPR013529 IPR013781 IPR017853 | |
| Gene3D | G3DSA:2.60.40.1180 G3DSA:3.20.20.80 | |
| KO | K12308 | |
| Pfam | PF02449 PF08533 PF08532 | |
| PIRSF | PIRSF001084 | |
| SUPFAM | SSF51445 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0003824 |
catalytic activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004565 |
beta-galactosidase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004565 |
beta-galactosidase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004565 |
beta-galactosidase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004565 |
beta-galactosidase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004565 |
beta-galactosidase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0005975 |
carbohydrate metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0008152 |
metabolic process |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
| GO:0009341 |
beta-galactosidase complex |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
| GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0016798 |
hydrolase activity, acting on glycosyl bonds |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0043169 |
cation binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0043169 |
cation binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
| GO:0004565 |
beta-galactosidase activity |
IMP: Inferred from Mutant Phenotype |
F |
LacR is known to be a regulator for lacA activity. Figure 2 shows that when mutant lacR strains are utilized, beta galactosidase activity is up regulated as long as there are no mutations in lacA. Mutant lacA strains results in decreased or no beta galactosidase activity showing that it is necessary for it to occur. |
complete | |||
| GO:0004565 |
beta-galactosidase activity |
IGI: Inferred from Genetic Interaction |
|
F |
Figure 1 shows that lacR is responsible for regulating beta-galactosidase activity and the lacR1 mutation upregulates beta-galactosidase activity. Figure 2 shows that when the same mutation is applied along with the lacA17 mutation, beta-galactosidase activity decreases showing that lacA is directly responsible for beta-galactosidase activity and is regulated by lacR. |
complete | ||
| GO:0004565 |
beta-galactosidase activity |
IMP: Inferred from Mutant Phenotype |
F |
Figure 3 presents a wild type plate that has grown blue colonies as a result of beta-galactosidase activity. When lacA is interrupted by the insertion of the CAT cassette as in the second plate shown, this expression is eliminated resulting in plain white colonies. |
complete | |||
| edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Daniel RA et al. (1997) Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR. J Bacteriol 179: 5636-8 PubMed GONUTS page
- ↑ 2.0 2.1 Errington J & Vogt CH (1990) Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator. J Bacteriol 172: 488-90 PubMed GONUTS page
- ↑ Shipkowski S & Brenchley JE (2006) Bioinformatic, genetic, and biochemical evidence that some glycoside hydrolase family 42 beta-galactosidases are arabinogalactan type I oligomer hydrolases. Appl Environ Microbiol 72: 7730-8 PubMed GONUTS page
bc |
c cont.h |
h cont.m |
