AZOVI:NIFL

Species (Taxon ID)	Azotobacter vinelandii. (354)
Gene Name(s)	nifL
Protein Name(s)	Nitrogen fixation regulatory protein
External Links
UniProt	P30663
EMBL	X64832 Y00554
PIR	S19883
PDB	2GJ3
PDBsum	2GJ3
ProteinModelPortal	P30663
SMR	P30663
IntAct	P30663
MINT	MINT-107527
EvolutionaryTrace	P30663
GO	GO:0005524 GO:0050660 GO:0000155 GO:0009399 GO:0006355 GO:0006351
Gene3D	3.30.565.10
InterPro	IPR003594 IPR014285 IPR001610 IPR000014 IPR000700 IPR013767 IPR004358 IPR005467
Pfam	PF02518 PF00989
PRINTS	PR00344
SMART	SM00387 SM00086 SM00091
SUPFAM	SSF55785 SSF55874
TIGRFAMs	TIGR02938 TIGR00229
PROSITE	PS50109 PS50113 PS50112

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
involved_in	GO:0006355	regulation of transcription, DNA-templated	PMID:8700899	ECO:0000314	direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
involved_in	GO:0018106	peptidyl-histidine phosphorylation	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0004673	P		Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR013767	P		Seeded From UniProt	complete
involved_in	GO:0007165	signal transduction	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR014285	P		Seeded From UniProt	complete
involved_in	GO:0009399	nitrogen fixation	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR014285	P		Seeded From UniProt	complete
involved_in	GO:0016310	phosphorylation	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004358	P		Seeded From UniProt	complete
	GO:0030528	transcription regulator activity	PMID:23056386	ECO:0000315			F		Figure. 3 N-terminal truncations affect the ability of NifL to regulate transcriptional activation by NifA	complete CACAO 8589
enables	GO:0016772	transferase activity, transferring phosphorus-containing groups	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR004358	F		Seeded From UniProt	complete
enables	GO:0004673	protein histidine kinase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:2.7.13.3	F		Seeded From UniProt	complete
enables	GO:0016740	transferase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0808	F		Seeded From UniProt	complete
involved_in	GO:0016310	phosphorylation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0418	P		Seeded From UniProt	complete
enables	GO:0000166	nucleotide binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0547	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0067	F		Seeded From UniProt	complete
	GO:0006355	regulation of transcription, DNA-dependent	PMID:8700899	ECO:0000314			P		Figure 2:NIFL is a regulatory protein that directs the transcriptional activities of nitrogen fixation genes. It does this by interacting with NIFA, which is an enhancer binding protein. Since previous research has shown that NIFL is a flavoprotein, this gene gets reduced in the presence of sodium dithionite. Transcription in inhibited when NIFL is oxidized. Transcriptional activiation can be measured by looking at the formation of open promoter complexes. This figure shows that sodium dithionite did not affect NIFA since the open promoter complexes were formed with or without the addition of sodium dithionite. This figure also shows that with the addition of sodium dithionite to NIFL and NIFA together, which would mean that NIFL is reduced, open promoter complexes were able to be formed. On the other hand, without sodium dithionite, NIFL is oxidized, and no open promoter complexes could be formed.	complete CACAO 2693
involved_in	GO:0009399	nitrogen fixation	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0535	P		Seeded From UniProt	complete
enables	GO:0016301	kinase activity	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0418	F		Seeded From UniProt	complete
involved_in	GO:0000160	phosphorelay signal transduction system	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0902	P		Seeded From UniProt	complete
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